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The Effect of Honey as a Treatment for Oral Ulcerative Lesions: A Systematic Review

  • Maddison Hunter1,2,
  • Jane Kellett1,
  • Nathan M. D’Cunha1,
  • Kellie Toohey1,2,3,
  • Andrew McKune1,3,4 and
  • Nenad Naumovski1,*
 Author information
Exploratory Research and Hypothesis in Medicine 2020;():-

DOI: 10.14218/ERHM.2019.00029


Background and objectives

A healthy oral environment features a rapid turnover rate of epithelium cells capable of regeneration and repair, with the oral epithelium contributing as a physical barrier and immune defense. However, the oral cavity can be subjected to unique damage, such as ulcerations. Honey is reported as a therapeutic agent for wound healing, due to its antioxidant, antibacterial and anti-inflammatory properties.


A systematic review was performed following the PRISMA 2015 Guidelines, to assess the efficacy and safety of the therapeutic use of honey in the oral cavity. Four electronic databases were searched (PubMed, Cochrane Library, Scopus, and Web of Science) for randomized controlled trials examining the effect of honey on oral cavity conditions.


In total, 2,832 records were identified, and after applying exclusion criteria, 13 studies were included. Honey was applied topically throughout, for chemotherapy or radiotherapy-induced oral mucositis (n = 11), dental wounds (n = 1), and recurrent aphthous stomatitis (n = 1), all of which are ulcerations with different pathologies. In the majority of studies (12/13), honey reduced the severity and/or duration of the condition compared with control groups (all p<0.05). However, a group treated with Manuka honey (n = 1) experienced adverse effects and considerable participant attrition.


Honey is an effective treatment for a range of oral ulcerative conditions. Future research should focus on compositional analysis of honeys to determine those with optimal beneficial properties, and whether Manuka honey is safe to use in the oral cavity.


Honey, Oral health, Ulcer, Oral cavity


The oral cavity consists of a range of structures, including the teeth, tongue, gingiva, and hard and soft palettes, with the latter being composed of stratified squamous cells.1 A healthy oral environment is characterized by a rapid cellular turnover rate, with the soft and hard palettes averaging between 14 and 24 days, respectively.2 One of the main purposes of the rapid turnover of the oral epithelium is as a defense mechanism, reducing the rate of pathogenic microbial colonization by removing the region they bind to.2 It also serves multiple additional functions, such as a physical barrier and immune defense to potential pathogens and toxins and in providing an environment that is optimal for the established healthy microbiome.1

The turnover of the mucosal cells in the oral cavity is dependent on a balance between cell differentiation and desquamation, which primarily acts as a pathogen defense mechanism.3 However, this can become unbalanced and lead to the development of several conditions, including hyperplasia and dysplasia, and a reduced rate of proliferation, which can lead to the development of ulcerations.2 Additionally, the oral mucosa is subject to masticatory and abrasive damage, with this oral epithelium disruption having the possibility of leading to the development of oral diseases1; some of these common oral conditions include dental caries, denture wounds, and ulcerations.4

The treatment of adverse health conditions in the oral cavity is focused on therapies that simultaneously eradicate pathogenic microbial growth, stimulate the wound healing process, and reduce the sensation of pain. One possible treatment option that fulfils these criteria is honey, which has been demonstrated to be effective in the treatment of a range of wounds, such as burns and ulcers.5 These potentially beneficial effects can be attributed to its antioxidant, antibacterial, and anti-inflammatory properties.57

The composition of honey, in particular the exact proportions of each of its constituents, is dependent on the floral source of the pollen used to produce the honey.8 Overall, honey is a mixture of several different categories of compounds, including polyphenols, ascorbic acid, carotenoids, organic acids, enzymes and other proteins,9 all of which contribute to honey being a viable nutritional source of antioxidants.10 Honey displays strong antioxidant activity and has the ability to reduce the effects of oxidative reactions, which produce free radicals and reactive oxygen species (ROS).8 In addition, honey is also reported to exhibit strong antibacterial effects, which can be attributed to its physicochemical properties, such as high osmolarity (due to high sugar levels)5 and relatively low pH11 due to the presence of several organic acids. Additionally, activation of glucose oxidase during the dilution of honey causes the production of hydrogen peroxide from glucose metabolism, providing an undesirable environment for growth and proliferation of bacterial cells12 and potentially mediating the wound healing process.13

The concomitant effects of the antioxidant and antibacterial properties of honey also contribute to its anti-inflammatory effects of reducing excessive inflammation, possibly resulting in a wound-healing effect.6 As the presence of ROS has been determined to lead to the production of inflammation,14 the antioxidant activity of honey can also contribute to a reduction of an excessive inflammatory response.7 Moreover, honey’s capability to prevent the development of bacterial infections (through providing an environment that cannot support bacterial growth and proliferation) can further assist with the inhibition of inflammation.5 Furthermore, the application of honey to a wound has been demonstrated to stimulate the production and release of pro-inflammatory cytokines that assist in the wound healing process, such as interleukin-1 and tumor necrosis factor-alpha.5 The topical application of honey to various injured tissues has also been shown to stimulate wound repair through the stimulation of growth of epithelial cells, reduction of edema, and wound debridement.6,7

It is well established that an individual’s oral health impacts their quality of life.4,15,16 Poor oral health is shown to affect physical and psychological wellbeing through condition-related reductions in functionality, including the inability to consume adequate nutrition and communicate, undesirable effects on physical appearance, in addition to causing pain.15 These conditions, particularly those related to the soft and hard palette such as ulcerations, have been reported as treated using various types of honey in several different clinical populations.1719 These collective data suggest honey as a reliable food product, being both financially affordable and readily available, that could be used in the treatment of inflammatory and microbial damage of the epithelial lining of the oral cavity. Therefore, this systematic review aimed to assess the efficacy of honey as a therapeutic agent used for the treatment of epithelial damage due to the inflammatory and microbial response in the oral cavity. Additionally, this review will aim to evaluate the safety of honey in different clinical populations.


This systematic review has been completed in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 201520 Guidelines and was registered in the International Prospective Register of Systematic Reviews (PROSPERO Number: CRD42019128480). The systematic review was completed following the Population, Intervention, Comparator, Outcomes and Setting (PICOS) approach as follows:

Population: Human populations with an existing oral health condition, or at risk of developing an oral health condition.

Intervention: Randomized controlled trials utilizing topically-applied undiluted honey from any floral source as a treatment or preventative agent without the concurrent use of other treatments. Only trials containing sample sizes over 12 participants were included; however, there was no time limit placed on study duration.

Comparator: Human comparators being treated with a placebo, control or routine standard oral care.

Outcomes: Effects of honey treatment on severity of oral health conditions, improvements in healing time, and any adverse effects to participants caused by the use of honey during and following the intervention protocol.

Setting: Any.

Search strategy

The following four electronic databases were searched; Scopus, PubMed, Cochrane Library and Web of Science, for manuscripts published since journal inception until June 2019. Furthermore, searches were also conducted to identify any new publications prior to submission of this manuscript (end of October 2019). The searches were limited to studies that were conducted on humans, were randomized controlled trials, and were published in peer-reviewed journals in the English language. The reference lists of included articles were also searched to locate any additional articles relating to the topic. The search terminology used included “honey*” and “oral”, “mouth”, “intraoral”, “cavity”, “bucc*”, “tooth”, “orthodont*”, “saliv*”, and “disease”, “condition”, “lesion”, “wound”, “carie*”, “plaque”, “ulcer”, “bacter*”, “micro”, “count”, with the terms AND and OR between every term.

Selection criteria

Studies were included if they were original research, randomized controlled trials published in English and in peer-reviewed journals. Articles were only considered if they utilized undiluted honey without any other interventions as a treatment against a pre-existing condition of the mouth or as a preventative agent in a group more susceptible to developing an oral condition. Furthermore, articles were required to have a control or placebo group as a comparator. Any articles that did not meet these criteria were excluded from this review. There were no time limitations posed on the duration of the studies, as oral conditions have different durations and pathological severities. For the purpose of this systematic review, a condition of the mouth was defined as a symptomatic problem unique to the oral cavity, such as ulcers or dental wounds.

Data extraction

All articles identified in the initial electronic search were imported into an EndNote library (vX8.2; Clarivate Analytics, USA) and duplicates removed. We excluded articles that did not investigate honey or oral health conditions. Following this, articles were excluded based on study design, and only if pure undiluted honey was utilized in the absence of any other treatments, with the exception of patient-required analgesics.

Two authors (MH and JK) independently screened each of the articles based on the selection criteria. The titles were screened to determine relevance, followed by screening of abstracts to determine studies suitable for full-text analysis. Any disagreements regarding the selection criteria were resolved through discussion until consensus was reached or in consultation with a third author (NN). At this stage, the reference lists of the articles considered for full-text review were searched for additional articles. The extraction of relevant data was completed by two authors (MH and JK) working independently in predesigned tables including the information on author name, year of publication, condition assessed, honey application protocol, control or placebo, number of participants, and outcomes.

Assessment of bias

The risk of bias was independently assessed according to the Cochrane Collaboration’s Tool for Assessing Bias, which utilizes the domains of selection bias through random sequence generation and allocation concealment, performance bias through blinding of participants and personnel, detection bias through blinding of outcome assessment; attrition bias through the addressing of any incomplete data, reporting bias through selective reporting, and any other sources of bias.21 The risk of bias was assessed independently by two authors (MH and JK), with a third author (NN) introduced in the case of any disagreements, where appropriate.

Data analysis

Due to the broad inclusion criteria used for this review to include a number of ulcerative lesions, which includes a variety of population groups and intervention protocols, this review consists of considerable heterogeneity. As such, a meta-analysis was not deemed appropriate.


Study selection

The initial search of the electronic databases (Fig. 120) resulted in 2,838 articles, with the secondary searches identifying 1 article. Following removal of articles due to duplicates (n = 1,181), study design (n = 115), absence of honey investigation (n = 1,416) and absence of oral health intervention (n = 80), 46 studies were subjected to full text-analysis. Articles were further excluded due to use of diluted honey with or without the use of additional therapies (n = 14), unsuitable oral health conditions (n = 12) and study design (n = 8), with 13 studies fitting the inclusion criteria.2234 An article investigating the use of honey to assist in the recovery of dental extraction surgery35 was considered but it was determined that since this wound is due to surgery, and not a condition unique to the oral cavity, this article was excluded.

Search strategy and article selection process according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) Guidelines.
Fig. 1  Search strategy and article selection process according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) Guidelines.20

Study characteristics

The characteristics of the included studies are presented in Table 1.2234 The total number of participants recruited in the included studies was 634, with sample sizes ranging from 20 to 106 participants. Of these, 11 studies investigated oral mucositis (OM) in 579 cancer patients,2232 1 investigated 35 participants with denture-induced ulcers,33 and 1 investigated 20 participants with recurrent aphthous stomatitis (RAS).34 Although the selection criteria of this review was designed to include a broad range of oral health conditions, the intervention was common across studies, with pure, undiluted honey being applied topically to affected areas in the mouths of patients in the treatment groups. The type of honey and their floral sources used in the included studies was largely unspecified26,28,30,33,34; however, two studies stated that the honey was sourced from a commercial supermarket.23,27 Five of the studies identified the local flora that was used in the production of the honey used,22,24,29,31,32 and one study specified their use of Manuka honey.25

Table 1

Summary of included study characteristics

Abdulrhman et al. (2012)22Lymphoblastic leukemia receiving chemotherapy; Grade 2 and 3 OM; n = 90Group 1: 0.5 g/kg applied topically to the affected oral mucosa three times daily until healing or for 10 days and routine oral care; n = 30Group 2: 0.25 g/kg of 4:2:1 mixture of honey, olive oil-propolis extract and beeswax (HOPE) applied topically three times daily until healing or for 10 days and routine oral care; n = 30
Group 3: benzocaine 7.5% gel applied to oral mucosa three times daily and routine oral care; n = 30
Recovery time reduced for Grade 2 OM (Group 1 p < 0.05)
Recovery time reduced for Grade 3 OM in Group 1 and Group 2 (p < 0.01)
Combining Group 2 and Group 3, honey had fastest healing
HOPE: burning sensation
Al Jaouni et al. (2017)23Pediatric cancer receiving chemo/radiotherapy; n = 40Honey applied topically to oral mucosa, followed by alkaline saline rinse four to six times daily and routine oral care; n = 20Routine oral care; n = 20Reduced Grade 3 and 4 OM in honey group (20% vs. 55%) and reduced hospitalization
(p < 0.05), increased bodyweight, delayed onset, decreased pain
Biswal et al. (2003)24Head neck cancer patients receiving radiotherapy
n = 40
20 mL honey applied to mouth before, immediately after, and 6 h after radiotherapy; n = 20Routine oral care; n = 20Difference in G3 and G4 mucositis was 20% (treatment) and 75% control (p < 0.00058)
Ceylan et al. (2010)*33New denture wearers; n = 35Honey applied to wound 3 times daily for 3 days; n = 25Salt water rinse three times daily; n = 10Difference between groups in the patients’ assessment (p < 0.05)
Honey relieved symptoms earlier than control
Halim et al. (2013)34Minor recurrent aphthous stomatitis patients; n = 20Honey applied three times daily for 5 days; n = 10Salicylate gel applied three times daily for 5 days; n = 10No difference between honey and salicylate gel for pain score and ulcer size (p > 0.05); observed improvement in pain and ulcer size
Hawley et al. (2014)25Head and neck cancer patients receiving radiotherapy; n = 1065 mL honey applied four times daily following saline rinse; fluoride treatment at bedtime; n = 545mL placebo gel applied four times daily following saline rinse; fluoride treatment at bedtime; n = 52No difference between groups for OM Grade 3 (p = 0.77)
Honey and placebo contributed to nausea; 57% treatment and 52% placebo dropout rate
Howlader et al. (2019)26Head and neck cancer patients receiving chemo/radiotherapy; n = 4020 mL honey applied to mouth 15 min before and after, and 6 hours after radiotherapy; n = 20Saline rinsed in mouth 15 min before and after radiotherapy; n = 20No difference (p > 0.05) in mucositis grade weeks 1–4 (radiotherapy administered); improvements in 4th week post radiotherapy (p = 0.0001)
Jayachandran et al. (2012)27Oral cancer patients receiving radiotherapy; n = 60Group 1: 20 mL honey rinsed 15 min before and after, and 6 h after radiotherapy; n = 20Group 2: 15mL 0.15% benzydamine hydrochloride rinsed 15 min before and after, and 6 h after radiotherapy for 5 min; n = 20
Group 3: 20 mL 0.9% saline rinsed 15 min before and after, and 6 h after radiotherapy for 5 min; n = 20
Delayed onset for Group 1 and reduced severity compared to Group 2 and Group 3 (p < 0.05)
Jayalekshmi et al. (2016)28Head and neck cancer patients receiving radiotherapy; n = 2815 mL honey rinsed 15 min before and after, and 6 h after radiotherapy; n = 1415 mL water rinsed 15 min before and after, and 6 h after radiotherapy; n = 14Reduction in OM in week 4 (p < 0.05) and 5 and 6 (p < 0.01)
Control: 61.54% developed Grade 3 OM. Treatment: 9.09% developed Grade 3 OM
Khanal et al. (2010)29Oral cancer patients receiving radiotherapy; n = 4020 mL honey rinsed 15 min before and after radiotherapy, and at bedtime; n = 2020 mL lignocaine gel rinsed 15 min before and after radiotherapy, and at bedtime; n = 20Honey associated with less severe OM (p < 0.0001)
Group 1: 1/20; Group 2: 15/20 developed intolerable OM
Maiti et al. (2012)*30Head and neck cancer patients receiving chemo/radiotherapy; n = 5520 mL honey rinsed 15 min before and after radiotherapy, and at bedtime; n = 28Standard oral care; n = 27Honey delayed onset in about 80%; 63% in control group had severe OM
Grade 3 OM: 5/28 in intervention arm, 11/27 in control. Grade 4: 1/28 in intervention arm, 5/27 in control
Motallebnejad et al. (2008)31Head and neck cancer patients receiving radiotherapy; n = 4020 mL honey rinsed 15 min before and after, and 6 h after radiotherapy; n = 2020 mL 0.09% saline rinsed before and after radiotherapy; n = 20Control group had treatment interruptions
OM score lower (p < 0.001) in treatment group;
20% in intervention group did not develop OM symptoms
Rashad et al. (2009)32Head and neck cancer patients receiving chemo/radiotherapy; n = 4020 mL honey rinsed 15 min before and after, and 6 h after radiotherapy; n = 20Standard oral care; n = 20Reduced severity in honey group: 3/20 Grade 4 (honey arm), 9/20 Grade 4 (control arm) (p < 0.05)

In the included articles, participants were instructed to maintain good oral hygiene (n = 6)22,2426,31,32 or prescribed the use of antibacterial, antifungal or analgesic solutions (n = 3).22,23,32 However, this was not specified in six studies.2730,33,34 For trials investigating OM (n = 11), the studies investigated head and neck cancer patients in adult populations2432 and pediatric patients receiving treatment for a range of cancers, including leukemia.22,23 Participants with radiotherapy-induced OM were included in six studies,24,25,2729,31 with 1 study having investigated chemotherapy-induced OM22 and 4 having determined the effect of honey on concurrent chemo-radiotherapy-induced OM.23,26,30,32 These studies utilized routine oral care,23,24,30,32 saline rinses of different concentration ranges (0.9%,27 0.09%,31 and unspecified26), water,28 and a variety of anesthetic and analgesic solutions (7.5% benzocaine gel,22 15% benzydamine hydrochloride,27 lignocaine gel29) serving as controls, while only one study utilized a placebo gel25 and another developed their own mixture.22


In a study by Biswal et al.,24 patients receiving radiotherapy for head and neck cancer were provided with 20 mL of honey to smear on the inside of their mouths, and instructed to swallow it slowly in order to coat both the oral and pharyngeal mucosa, with application occurring 15 min before and after they received radiotherapy and 6 h after receiving radiotherapy, for the duration of the treatment cycle. This study design was also replicated by other studies included in this review,27,2932 with Jayalekshmi et al.28 and Howlader et al.26 with slight modifications, namely use of 15 mL of honey28 and consumption of additional honey to potentially contribute to serum antioxidant levels.26

The remaining studies investigating OM utilized study designs similar to those above, topically coating the oral cavity with honey; however, the amounts of honey used and timing of application were different. In trials by Al Jaouni et al.23 and Hawley et al.25 durations were similar of treatments used to Biswal et al.24 and participants were followed throughout the duration of their oncology treatments. In addition, Hawley et al.25 maintained the intervention for 7 days following final radiotherapy treatment, with all studies applying the honey intervention four times daily. The volumes of honey and subsequent saline rinse were not specified by Al Jaouni et al.23 but Hawley et al.25 specified the prescription of 5 mL of applied honey followed by a fluoride rinse. Abdulrhman et al.22 included participants with established OM using undiluted honey (0.5 g/kg) and having two control groups (receiving a custom-made mixture (placebo) or an analgesic control). All groups applied their mixtures three times daily for 10 days or until healing of ulceration.

The study by Ceylan et al.33 investigated the use of honey in the treatment of traumatic ulcers that were caused by newly fitted dentures in 35 healthy, previously edentulous participants. Of these, 25 participants were provided with honey and instructed to smear directly onto the ulcers three times daily for 3 days following denture fitting. The control group (n = 10) were provided with a saline rinse to use via the same protocol as the treatment group. The ulcer areas were measured in all participants using planimetry on the first and third day following initial denture fitting.

The study by Halim et al.34 investigated the effectiveness of honey in the treatment of RAS in healthy adults. Following an initial assessment, participants in the treatment group (n = 10) were given honey and control groups (n = 10) were given salicylate gel, a standard prescribed treatment for RAS, to apply to their ulcers 3 times daily for 5 days. Ulcer size was determined using calipers, and a visual analog score was used to assess pain before and after the intervention.

Study results

The majority of studies investigating the use of honey in the treatment of OM in an oncology population were successful in alleviating negative symptoms, including reducing the severity or duration of the condition (all p<0.05) (Table 1).2224,2629,31,32 In these studies, OM was assessed using a grading scale, with Grade 1 representing less severe cases and Grade 4 being the most severe. In these studies, the onset of OM was delayed in the honey-treatment group compared to the control group, and fewer participants in the honey group of each study developed higher gradings of the condition. One OM study did not report any significant changes from pre- to post-treatment nor any difference between the two groups (treatment and control); however, there was a self-reported observed difference in the OM.30 All of these studies utilized standard honey from no particular floral source, with no adverse reactions reported throughout the duration of the honey intervention, excepting one study31 that reported the exclusion of participants who reported a stinging sensation, though the number of exclusions and effect on the study was not disclosed. A single study by Hawley et al.25 utilized Manuka honey and reported no significant difference in the severity or duration of OM between the honey and placebo groups (p = 0.77); however, this study experienced considerable dropout rate (57% in the honey group, 52% in the placebo group), mainly attributed to the sensation of nausea caused by the application of the honey.

It was determined that honey significantly (p value unreported) relieved the symptoms of the denture-induced ulcers earlier than in the control group, and (as assessed by a visual analogue score) reduced pain, with no adverse effects.33 Finally, while no participants with RAS reported adverse effects, no significant difference was found between the honey group and control group of a currently utilized treatment for ulcer size (p = 0.879) and pain (p = 0.514); however, self-reported improvements were observed.34

Risk of bias in included studies

The risk of bias was assessed according to the Cochrane Risk of Bias Tool and is outlined in Table 2.2234 The risk of selection bias through allocation concealment was high for the included studies, which was due to placebo products being utilized by 2/13 studies.22,25 Additionally, Jayalekshmi et al.28 reported the use of similar bottles for both their treatment and control groups, resulting in a low risk of selection bias. Similarly, the performance bias was high or unclear, which was attributed to the common utilization of controls rather than a placebo. The detection bias for the included studies was relatively unclear but there was an overall low risk of attrition and reporting bias (Table 2). No other bias was detected.

Table 2

Assessment of risk of bias in included studies.

StudyRandom sequence generation (selection bias)Allocation concealment (selection bias)Blinding of participants and personnel (performance bias)Blinding of outcome assessment (detection bias)Incomplete data addressed (attrition bias)Selective reporting (reporting bias)Other bias
Abdulrhuman et al. (2012)22LowLowUnclearUnclearLowLowUnclear
Al Jaouni et al. (2017)23UnclearHighHighUnclearLowLowUnclear
Biswal et al. (2003)24LowHighUnclearUnclearLowLowUnclear
Ceylan et al. (2010)33UnclearHighUnclearUnclearLowLowUnclear
Halim et al. (2013)34UnclearHighUnclearUnclearLowLowUnclear
Hawley et al. (2014)25LowLowLowLowLowLowUnclear
Howlader et al. (2019)26LowHighHighLowLowLowUnclear
Jayachandran et al. (2012)27UnclearHighUnclearUnclearLowLowUnclear
Jayalekshmi et al. (2016)28LowHighHighUnclearLowLowUnclear
Khanal et al. (2010)29LowHighHighLowLowLowUnclear
Maiti et al. (2012)30UnclearHighUnclearUnclearLowLowUnclear
Motallebnejad et al. (2008)31UnclearHighLowLowLowLowUnclear
Rashad et al. (2009)32UnclearHighHighUnclearLowLowUnclear


Effectiveness of honey

The primary aim of this review was to determine the effectiveness of topically-applied honey in the treatment of a range of oral health conditions. Of the 13 studies included in this review, 10 reported statistically significant reductions in the severity of the oral health condition compared to a control group, including 9 studies investigating OM2224,2629,31,32 and 1 investigating denture wounds.33 While one study did not report any significance30 and another reported no significant difference in the improvement of RAS,34 the oral condition was improved in both studies, demonstrating that honey was just as effective as the standard prescribed treatments for these conditions. Only one study, by Hawley et al.,25 did not report any improvements in oral condition.

It has been proposed that honey possesses wound healing abilities due to its antioxidant and antibacterial properties,57 which includes the ability to heal ulcerations. All conditions included in this review culminated in the development of an oral ulcer, which had formed due to the loss and necrosis of epithelial tissue and resulting in breaks in the skin or mucous membrane36; each condition formed due to different pathologies. Oral mucositis is a condition that develops as a toxicity during oncology treatments (chemotherapy and radiotherapy) and is characterized by inflammation, erythema, microbial colonization, and ulceration.37 Similarly, RAS is a condition of unknown cause that affects the lining of the mouth, causing erythema, burning and ulceration,34 and denture-induced ulcers are caused by the introduction of ill-fitting dentures causing abrasive damage to the lining of the mouth, resulting in ulcerative lesions.33

The antibacterial and antioxidant properties of honey contribute to honey’s wound-healing abilities. In the case of OM, the initiation phase is characterized by the production of ROS,37 which could potentially cause further damage to the affected areas. A study investigating participants with RAS determined that those with the condition had significantly lower salivary antioxidant levels compared to healthy controls,38 demonstrating that the ulcerations potentially produced ROS, causing a reduction in the antioxidant compounds present in the mouth. This highlights a possible mechanism for the effectiveness of honey as a treatment for the ulcerations discussed in this review. Potentially, the presence of the antioxidant compounds provided by the topically-applied honey can reduce the effects of ROS activity during the development of ulcerations. Furthermore, the consumption of honey increases total systemic plasma antioxidant levels, and swallowing of the topically-applied honey may contribute to longer lasting antioxidant effects,10 further contributing to the wound healing process.

Adverse effects

The secondary aim of this systematic review was to determine any adverse effects during the topically-applied honey interventions. Only one of the included studies reported an adverse effect due to the honey intervention.25 Namely, Hawley et al.25 reported considerable dropouts due to nausea, which could be attributed to the treatments being associated with radiotherapy; however, it was reported that the Manuka honey that was used worsened nausea symptoms and encouraged a retching motion. A study by Bardy et al.39 also used Manuka honey and reported a low compliance rate due to the honey being difficult to use, along with an undesirable taste and texture. A study by Parsons et al.40 initially had to be discontinued due to the Manuka honey not being well-tolerated by participants, and in that study all participants withdrew due to a perceived stinging pain and nausea following application. Interestingly, Abdulrhman et al.22 reported no adverse side effects in the honey intervention arm of their study, which used honey from a different floral source to Manuka honey; however, in their comparative group using their own formulation, discomfort was reported, which was proposed to be due to the addition of propolis. The studies investigating denture wounds33 and RAS34 did not report any adverse effects. This further supports evidence that the application of honey to the inside of the oral cavity is effective in reducing the pathogenic bacteria that cause dental caries, and has a positive effect on plaque levels and gingivitis.17,41,42 These findings demonstrate that the use of honey in future studies is safe for the dental health of participants. It was also proposed that similar trials comparing two intervention groups of Manuka and conventional honey should be completed18; however, given the evidence of the adverse effects of Manuka honey, this may not be feasible.

Issues with use and development of placebo

The majority of the studies included in this review (n = 12) utilized a control or ‘standard’ prescribed care in the place of a placebo. Due to the nature and progression of oral health conditions included in this review, particularly OM in oncology patients, it would be unethical to provide no treatment to the control group, resulting in most studies in this review receiving standard care or a commonly-used treatment. This is also partially reflected by the reported bias (Table 2), where many studies were graded as having a “high risk” of selection and performance bias, with the exception of the study by Jayalekshmi et al.28 who utilized similar packaging for their honey treatment and control.

If a placebo were to be used in future studies to assess the effectiveness of honey as a topical treatment, it would be best practice to use products which share certain physicochemical properties with honey to support undetectability by participants, such as its properties of osmolarity and acidity.31 The use of such a placebo could have confounding effects, as the physicochemical properties of honey, including its osmolarity, contribute to honeys wound-healing abilities.19 One such example is brought forth by the study by Bardy et al.,39 which had been excluded (during full-text review) from our review due to not having used undiluted honey. In that study, golden syrup which has a similar appearance and texture to honey, was assigned as a placebo alongside a Manuka honey treatment for OM. While there was no significant difference observed in the severity or duration of the OM between the treatment and control groups (p > 0.05), the pathogenic bacteria present in both groups was consistent with baseline throughout the study period, demonstrating that both honey and the placebo controlled the bacterial growth. This further supports the notion of the importance of osmolarity action (shared by the honey and golden syrup) on the antibacterial activity of honey.

The only study in this review that utilized a placebo was completed by Hawley et al.,25 where the product was developed to look like honey. Similar to the study by Bardy et al.,39 this study did not result in a significant reduction in the severity or duration of the OM experienced by participants; however, this could be attributed to the possible non-suitability of Manuka honey for this type of treatment, rather than the activity of the placebo. Additionally, a study by Abdulrhman et al.22 developed a honey-containing product, which they assessed as a treatment for OM alongside a treatment group that received pure, undiluted honey, in addition to the use of a standard care control (Table 1).

It is evident that the utilization of a placebo in studies of this nature is not practical, and so further care should be taken in future studies to ensure that both participants and researchers are blinded to the allocation of honey or control. This could potentially be achieved through disclosing that the intervention is designed to improve the oral health condition, while not specifying that honey is the treatment.

Compositional properties of used honeys

It is well established that several honey properties can contribute to its wound healing effects, including a combination of antioxidant, physicochemical and antimicrobial properties. However, the optimal levels of compounds or categories of compounds, including their interaction with each other, that can contribute to the observed beneficial health effects of honey, are largely unexplored. Four of the included studies, all investigating OM, completed in vitro analysis of the potential wound healing properties, including pH,22,24,31,32 moisture content,22,24,31,32 phytochemical profile,24,32 and microbiological analysis.24,31 However, none of the studies utilized these results in their discussion on the effectiveness of honey as a treatment. A trial of this nature should be completed to determine the individual in vitro wound healing properties of different types of honey, so that recommendations can be offered to participants experiencing these oral health conditions about what type of honey should be used to provide optimal benefits. Due to the overall lack of analysis of the honey utilized in these trials, it is difficult to draw conclusions about the best type/s of honey and the properties that are most appropriate for use in the treatment of these oral conditions.


The inclusion criteria for this review evaluated only articles that utilized pure, undiluted honey as a treatment, as the concurrent utilization of other compounds or medications has the possibility to contribute to any potential beneficial effect provided by just the honey in isolation. As a result, studies that utilized honey and no other treatments were included, though the use of standard care was allowed. It would be unethical to request that patients discontinue their normal oral care routines for the duration of an intervention, due to the potential impact on their dental health. Additionally, the conditions investigated in this review can cause considerable pain, which could lead to the requirement of the additional analgesics. This may provide a reduction in the effectiveness of the honey; however, the use of honey in isolation to routine oral care in interventions such as the ones included in this review is impractical.

Future research proposals

The adverse effects caused by Manuka honey highlighted in this review should be taken into consideration of future research for oral health conditions. Manuka honey contains relatively high levels of methylglyoxal (MGO), which has been identified to be unique to this type of honey and directly contributes to Manuka honey’s antibacterial abilities.43,44 This compound also occurs in humans as a by-product to metabolic processes, such as glycolysis45; however, it has been shown to exhibit potentially toxic side effects, including the ability to modify DNA and other macromolecules.46 Additionally, in healthy human cells, the tripeptide glutathione (GSH) is capable of suppressing the activity of MGO47; however, once a cell becomes damaged, such as during the oral conditions discussed in this review, there is a reduction in the expression of GSH.48 This can result in further damage, with GSH depletion leading to MGO accumulation.49 This is depicted in Figure 2, where, in a healthy oral cavity, GSH is able to suppress the activity of MGO, thereby preventing any cellular modifications. Although, when Manuka honey is applied to the damaged oral cavity, the reduced levels of GSH allow the levels of MGO to increase, and to potentially cause further cellular damage.

Effect of honey on the oral cavity.
Fig. 2  Effect of honey on the oral cavity.

(a) GSH present in the healthy oral cavity suppresses the activity of MGO from Manuka honey. (b) The use of honey does not cause adverse effects in a healthy oral cavity, and is effective in treating a damaged oral cavity. (c) Reduced levels of GSH in the damaged oral cavity do not suppress the activity of MGO from Manuka honey, with the MGO potentially causing further damage, and Manuka honey not being an effective treatment. GSH, tripeptide glutathione; MGO, methylglyoxal.

As previously discussed, one study included in this review reported considerable adverse effects following the topical application of Manuka honey.25 Studies of similar interventions also reported adverse effects,39,40 which could potentially be due to the proposed interactions between MGO and reduced levels of GSH. These adverse reactions were not observed in studies utilizing honey from other floral sources, with the topical application of honey to a damaged oral cavity being demonstrated to result in improvements to the oral condition (Fig. 2).

While Manuka honeys have demonstrated substantial potential wound healing abilities in vitro and in vivo,11,44,50 there is currently a lack of relevant clinical trials utilizing this product for wound healing treatments. Due to its compositional properties, it should be considered that Manuka honey be utilized in clinical trials for site-specific topical application in wound sites other than the oral cavity, such as a laceration or burn on the skin to test for effectiveness and tolerability. Similarly, further trials in the treatment of oral conditions or investigating alternative application sites could be completed to determine optimal levels of MGO that can be tolerated.


The use of honey as a therapeutic treatment of oral conditions has been identified to be effective in reducing the duration and severity of conditions in comparison to standard treatments and controls. Specifically, it has been demonstrated that honey is effective against a range of oral ulcers with multiple pathologies and, in particular, in the treatment of oral mucositis. Any adverse reactions that were reported throughout the duration of the included studies could be attributed to the type of honey used and were not due to the type of oral health condition, demonstrating that the topical application of honey, other than Manuka honey, is relatively safe for use across a range of oral health conditions. The use of honey for the treatment of oral mucositis should be considered for further research; however, control over honey composition should be taken into consideration, particularly phytochemical composition. The use of Manuka honey in the treatment of oral cavity conditions should be carefully considered before use, with future investigations required to determine its safety.



reactive oxygen species


preferred reporting items for systematic reviews and meta-analyses


population, intervention, comparator, outcomes and setting


oral mucositis


recurrent aphthous stomatitis









M. Hunter is supported by an Australian Government Research Training Program Scholarship. N. M. D’Cunha is supported by a Dementia Australia Research Foundation PhD Scholarship. All other authors have no sources of funding to declare.

Conflict of interest

All authors declare no conflict of interests.

Authors’ contributions

Study concept and design (MH, NN); data acquisition (MH, JK); data analysis (MH, JK, NN); drafting of the manuscript (MH); critical revision of the manuscript (MH, JK, NMD, KT, AM, NN); administrative, technical, and study supervision (NN).


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