Baicalin reduces the stemness potential of hepatoblastoma rather than hepatocellular carcinoma and improves its chemo-sensitivity
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Guo-Ping He1,
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Xiao-Zhi Liu2,
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Na Xue2,
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Tian Yu2,
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Yan-Xia Li2,
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Chun-Yan Zhang2,* and
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Hua-Qing Wang3,4,*
Author information
Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Central Laboratory, The Fifth Central Hospital of Tianjin, Tianjin 301617, China
Department of Oncology, Tianjin Union Medical Center of Nankai University, Tianjin 300121, China
The Institute of Translational Medicine, Tianjin Union Medical Center of Nankai University, Tianjin 300121, China
Correspondence to: Chun-Yan Zhang, Central Laboratory, The Fifth Central Hospital of Tianjin, Tianjin 301617, China. E-mail:
chunchun2746@126.com; Hua-Qing Wang, Department of Oncology, Tianjin Union Medical Center of Nankai University, Tianjin 300121, China. E-mail:
huaqingw@163.com
Abstract
Introduction
Hepatoblastoma (HB) is the most common malignant liver tumor in children. Although surgery and chemotherapy have greatly improved the survival rate of children, the emergence of chemotherapy resistance still threatens the survival of most patients. Baicalin (Ba) is a kind of flavonoid bioactive substance with strong anti-tumor effect, but the effect of Ba on HB remains to be explored. Therefore, studying the effect and mechanism of Ba on HB may provide new opportunities for improving chemotherapy resistance.
Objective
To explore the effect and mechanism of Ba on the anti-tumor effect and cisplatin sensitivity of HB cell line Hep G2 and hepatocellular carcinoma cell line Hep 3B.
Methods
The anti-tumor effects of Ba on Hep G2 and Hep 3B cells and its influence on cisplatin sensitivity were evaluated by phenotypic experiments; Western blotting and colony formation assay are used to detect the influence of Ba on the stemness potential of two cell lines; Finally, the effect of Ba on the sensitivity of Hep G2 tumor-bearing mice to cisplatin was further verified in vivo.
Results
Ba can significantly inhibit the malignant phenotype and stemness potential of Hep G2 in vitro, and increase its sensitivity to cisplatin, but it has no effect on Hep 3B. Further mechanism studies have shown that Ba can inhibit the clone ball formation of Hep G2 cells and down-regulate the expression of Oct4 and Sox2 proteins, but this effect has not been found in Hep 3B cell lines. Further in vivo verification showed that Ba increases the sensitivity of Hep G2 tumor-bearing mice to cisplatin.
Conclusion
Our study confirms the important role of Ba in HB chemotherapy resistance. Ba increases its chemotherapeutic sensitivity to cisplatin by reducing the stemness of HB cells, and this effect is selective for tumor types and has no effect on hepatocellular carcinoma.
Keywords
baicalin,
hepatoblastoma,
hepatocellular carcinoma,
cisplatin,
chemotherapy sensitivity,
Oct4,
Sox2
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Copyright © 2025 Authors.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 4.0 License (CC BY-NC 4.0), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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He GP, Liu XZ, Xue N, Yu T, Li YX, Zhang CY, et al. Baicalin reduces the stemness potential of hepatoblastoma rather than hepatocellular carcinoma and improves its chemo-sensitivity. Gastroenterol & Hepatol Res. 2021;3(3):13. doi: 10.53388/ghr2021-09-036.
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Article History
| Received |
Revised |
Accepted |
Published |
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September 12, 2021
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DOI
http://dx.doi.org/10.53388/ghr2021-09-036
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Gastroenterology & Hepatology Research
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eISSN 2703-173X